Hotspot

ycf3

LSC · 43548-45529 · 1982 bp

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Pi 0.00154
MarkerSeek score 53.90
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1982
variable_sites
9
pi
0.00154
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TGGATCTTCTCATGTCACGG TTTGTGGGAGCATTTACCCC 50.0 50.0 57.0 58.1 2125 bp 1.0 72.2
ycf3_p2 GGATCTTCTCATGTCACGGT TTTGTGGGAGCATTTACCCC 50.0 50.0 57.0 58.1 2124 bp 1.0 72.2
ycf3_p3 TTGGATCTTCTCATGTCACGG TTTGTGGGAGCATTTACCCC 47.6 50.0 57.7 58.1 2126 bp 1.0 69.9
ycf3_p4 TGGATCTTCTCATGTCACGG CCCTTGTTTGTGGGAGCATT 50.0 50.0 57.0 58.7 2131 bp 1.0 67.5
ycf3_p5 GGATCTTCTCATGTCACGGT CCCTTGTTTGTGGGAGCATT 50.0 50.0 57.0 58.7 2130 bp 1.0 67.5