Hotspot

rpl16

LSC · 83631-85042 · 1412 bp

Back to Yucca

Pi 0.0112
MarkerSeek score 58.40
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1412
variable_sites
38
pi
0.0112
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATCCTCAATACCCCTTTTGT AACCGTTTCTGTTTTTCCGT 40.9 40.0 57.1 56.4 1535-1553 bp 1.0 47.1
rpl16_p2 TCATCCTCAATACCCCTTTTGT ACCGTTTCTGTTTTTCCGTT 40.9 40.0 57.1 56.4 1534-1552 bp 1.0 47.1
rpl16_p3 TCATCCTCAATACCCCTTTTGT AAACCGTTTCTGTTTTTCCGT 40.9 38.1 57.1 57.1 1536-1554 bp 1.0 45.1
rpl16_p4 TCATCCTCAATACCCCTTTTGT AACCGTTTCTGTTTTTCCGTT 40.9 38.1 57.1 57.1 1535-1553 bp 1.0 45.1
rpl16_p5 TCATCCTCAATACCCCTTTTGTT ACCGTTTCTGTTTTTCCGTT 39.1 40.0 57.7 56.4 1534-1552 bp 1.0 44.5