Hotspot

psbA

LSC · 93-1154 · 1062 bp

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Pi 0.00152
MarkerSeek score 37.50
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
4
pi
0.00152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 GGTATGGCTCCTTCAACGAT GTGCTTGGGAGTCCTTGAAA 50.0 50.0 57.4 58.0 1149 bp 1.0 75.2
psbA_p2 TGGCTCCTTCAACGATTCTT GTGCTTGGGAGTCCTTGAAA 45.0 50.0 57.1 58.0 1145 bp 1.0 73.3
psbA_p3 GGTATGGCTCCTTCAACGAT TAGTACGTGTGCTTGGGAGT 50.0 50.0 57.4 58.4 1157 bp 1.0 72.3
psbA_p4 GGTATGGCTCCTTCAACGAT GTTAGTACGTGTGCTTGGGA 50.0 50.0 57.4 57.6 1159 bp 1.0 71.7
psbA_p5 GGTATGGCTCCTTCAACGAT AGTTAGTACGTGTGCTTGGG 50.0 50.0 57.4 57.5 1160 bp 1.0 71.7