Hotspot

ndhD

SSC · 118409-119911 · 1503 bp

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Pi 0.00577
MarkerSeek score 54.20
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
20
pi
0.00577
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 AGACTTTGCTTTCTGTCTCA AGAAGAACCCGTACTCGAGA 40.0 50.0 54.5 57.5 1601-1607 bp 1.0 49.7
ndhD_p2 AGACTTTGCTTTCTGTCTCA TACCGAGAAGAACCCGTACT 40.0 50.0 54.5 57.5 1606-1612 bp 1.0 49.6
ndhD_p3 AAGACTTTGCTTTCTGTCTCA AGAAGAACCCGTACTCGAGA 38.1 50.0 55.4 57.5 1602-1608 bp 1.0 48.5
ndhD_p4 AAGACTTTGCTTTCTGTCTCA TACCGAGAAGAACCCGTACT 38.1 50.0 55.4 57.5 1607-1613 bp 1.0 48.4
ndhD_p5 AGACTTTGCTTTCTGTCTCA ACCGAGAAGAACCCGTACTC 40.0 55.0 54.5 58.8 1605-1611 bp 1.0 47.0