Hotspot

accD

LSC · 59387-60883 · 1497 bp

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Pi 0.00293
MarkerSeek score 57.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1497
variable_sites
10
pi
0.00293
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ATAGGGGGACAGGAAGACTC ATGGGAGTTCTTGGTAGACA 55.0 45.0 57.5 55.5 1552-1578 bp 1.0 57.7
accD_p2 ATAGGGGGACAGGAAGACTC TGGGAGTTCTTGGTAGACAT 55.0 45.0 57.5 55.5 1551-1577 bp 1.0 57.7
accD_p3 TAGGGGGACAGGAAGACTCT ATGGGAGTTCTTGGTAGACA 55.0 45.0 58.6 55.5 1551-1577 bp 1.0 56.7
accD_p4 TAGGGGGACAGGAAGACTCT TGGGAGTTCTTGGTAGACAT 55.0 45.0 58.6 55.5 1550-1576 bp 1.0 56.7
accD_p5 ATAGGGGGACAGGAAGACTC AATGGGAGTTCTTGGTAGACA 55.0 42.9 57.5 56.3 1553-1579 bp 1.0 56.2