Hotspot

psaJ-rpl33

LSC · 69479-69968 · 490 bp

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Pi 0.00758
MarkerSeek score 35.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
490
variable_sites
7
pi
0.00758
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGGTCTTTAGCGGGTCTA CGGACATCTTTACCCTTAGCC 50.0 52.4 58.1 58.2 599-603 bp 1.0 71.1
psaJ-rpl33_p2 ATGGTTTGGGTCTTTAGCGG CGGACATCTTTACCCTTAGCC 50.0 52.4 58.2 58.2 604-608 bp 1.0 70.3
psaJ-rpl33_p3 TGGGTCTTTAGCGGGTCTAT CGGACATCTTTACCCTTAGCC 50.0 52.4 57.8 58.2 598-602 bp 1.0 70.3
psaJ-rpl33_p4 TTGGGTCTTTAGCGGGTCTA GGACATCTTTACCCTTAGCCA 50.0 47.6 58.1 57.1 598-602 bp 1.0 65.1
psaJ-rpl33_p5 GTTTGGGTCTTTAGCGGGTC CGGACATCTTTACCCTTAGCC 55.0 52.4 58.8 58.2 601-605 bp 1.0 64.9