Hotspot

ndhC-trnV-UAC

LSC · 51836-53443 · 1608 bp

Back to Yucca

Pi 0.00332
MarkerSeek score 61.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1608
variable_sites
14
pi
0.00332
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1662-1739 bp 1.0 81.6
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1744-1821 bp 1.0 79.2
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1675-1752 bp 1.0 78.9
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1661-1738 bp 1.0 78.8
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.4 57.8 1679-1756 bp 1.0 77.8