Hotspot

psbM-trnD-GUC

LSC · 30749-31808 · 1060 bp

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Pi 0.00429
MarkerSeek score 54.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1060
variable_sites
10
pi
0.00429
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC CCTCGCTGGGATTGTAGTTC 45.0 55.0 55.8 58.1 1188-1214 bp 1.0 63.0
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TACACCTCGCTGGGATTGTA 45.0 50.0 55.8 58.1 1192-1218 bp 1.0 62.3
psbM-trnD-GUC_p3 AGTGCGAGAATATTGACTTCCA CCTCGCTGGGATTGTAGTTC 40.9 55.0 57.5 58.1 1164-1190 bp 1.0 61.1
psbM-trnD-GUC_p4 AGTGCGAGAATATTGACTTCCA TACACCTCGCTGGGATTGTA 40.9 50.0 57.5 58.1 1168-1194 bp 1.0 60.4
psbM-trnD-GUC_p5 AGGAACTAGAATGAACAGTGCA CCTCGCTGGGATTGTAGTTC 40.9 55.0 57.4 58.1 1195-1221 bp 1.0 59.8