Hotspot

atpH-atpI

LSC · 14556-15047 · 492 bp

Pi 0.00465

MarkerSeek score 53.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 5
pi: 0.00465
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	551-556 bp	1.0	80.8
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.1	57.9	639-644 bp	1.0	80.3
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	576-581 bp	1.0	80.2
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	607-612 bp	1.0	80.0
atpH-atpI_p5	CGCAATACCTTCTACGGCTT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.1	57.9	664-669 bp	1.0	79.7