Hotspot

trnS-GCU-trnG-GCC

LSC · 8587-9758 · 1172 bp

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Pi 0.0113
MarkerSeek score 56.90
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1172
variable_sites
40
pi
0.0113
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 TGATCAGAGAGAGCGGAGAG CGAACGAATCACACTTTTACCA 55.0 40.9 58.0 57.3 1310-1329 bp 1.0 61.8
trnS-GCU-trnG-GCC_p2 AATCTGCCGCTTTAGTCCAC CGAACGAATCACACTTTTACCA 50.0 40.9 58.3 57.3 1243-1262 bp 1.0 60.2
trnS-GCU-trnG-GCC_p3 CAATCTGCCGCTTTAGTCCA CGAACGAATCACACTTTTACCA 50.0 40.9 58.3 57.3 1244-1263 bp 1.0 60.2
trnS-GCU-trnG-GCC_p4 GATCAGAGAGAGCGGAGAGA CGAACGAATCACACTTTTACCA 55.0 40.9 57.7 57.3 1309-1328 bp 1.0 60.1
trnS-GCU-trnG-GCC_p5 ATCAGAGAGAGCGGAGAGAG CGAACGAATCACACTTTTACCA 55.0 40.9 57.7 57.3 1308-1327 bp 1.0 60.0