Hotspot

rpl22

IRa · 157083-157460 · 378 bp

Back to Ypsilandra

Pi 0

MarkerSeek score 24.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: IRa
length_bp: 378
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCATTGGTGGGGGATAACCT	TACCGAGTCTGAAACCGAGT	50.0	50.0	58.0	58.1	509 bp	0.5	59.2
rpl22_p2	GTTATCCTGCGCTTGGAAGA	TACCGAGTCTGAAACCGAGT	50.0	50.0	58.0	58.1	1196 bp	0.5	59.0
rpl22_p3	TCATTGGTGGGGGATAACCT	TTTGCACACAATACCGCCTA	50.0	45.0	58.0	57.8	467 bp	0.5	58.5
rpl22_p4	GTTATCCTGCGCTTGGAAGA	TTTGCACACAATACCGCCTA	50.0	45.0	58.0	57.8	1154 bp	0.5	58.3
rpl22_p5	GATCCACTTGGCTACATCCG	TACCGAGTCTGAAACCGAGT	55.0	50.0	58.1	58.1	1023 bp	0.5	58.2