Hotspot

ndhF

SSC · 111621-113882 · 2262 bp

Back to Ypsilandra

Pi 0.00707

MarkerSeek score 60.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2262
variable_sites: 16
pi: 0.00707
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATTGGGAATTGGGAAGACT	TGGTGAATCGGAAACAATCA	40.0	40.0	54.5	55.3	2418-2444 bp	1.0	40.5
ndhF_p2	ATTGGGAATTGGGAAGACTT	TGGTGAATCGGAAACAATCA	40.0	40.0	54.5	55.3	2417-2443 bp	1.0	40.5
ndhF_p3	TGGGAATTGGGAAGACTTAAAGA	TGGTGAATCGGAAACAATCA	39.1	40.0	57.4	55.3	2415-2441 bp	1.0	40.5
ndhF_p4	AATTGGGAATTGGGAAGACTT	TGGTGAATCGGAAACAATCA	38.1	40.0	55.3	55.3	2418-2444 bp	1.0	40.5
ndhF_p5	ATTGGGAATTGGGAAGACTT	TGGTGAATCGGAAACAATCAA	40.0	38.1	54.5	56.1	2417-2443 bp	1.0	40.5