Hotspot

rpl23-rpl2

IRa · 154948-154965 · 18 bp

Back to Ypsilandra

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CCCGGGAAAGGGTAGAAGAA	TGCTCGGGGTAGAAGTTTTG	55.0	50.0	58.7	57.8	173 bp	1.0	73.3
rpl23-rpl2_p2	CCCGGGAAAGGGTAGAAGAA	GCTCGGGGTAGAAGTTTTGT	55.0	50.0	58.7	57.8	172 bp	1.0	73.3
rpl23-rpl2_p3	CCCGGGAAAGGGTAGAAGAA	GTGCTCGGGGTAGAAGTTTT	55.0	50.0	58.7	57.8	174 bp	1.0	73.3
rpl23-rpl2_p4	CCCGGGAAAGGGTAGAAGAA	TTGTCTATGGCTCCATTGCG	55.0	50.0	58.7	58.3	195 bp	1.0	72.0
rpl23-rpl2_p5	AGGGTAGAAGAATGGGACCT	TGCTCGGGGTAGAAGTTTTG	50.0	50.0	57.0	57.8	165 bp	1.0	71.3