Hotspot

rps15-ycf1

SSC · 125087-125564 · 478 bp

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Pi 0.0167

MarkerSeek score 55.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 478
variable_sites: 8
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCGAAGTAAGCTTACGTACC	CGGCAGTCGTTTCAGTATGT	47.6	50.0	58.1	58.3	655-668 bp	1.0	69.6
rps15-ycf1_p2	GCGAAGTAAGCTTACGTACCT	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.9	58.3	654-667 bp	1.0	69.5
rps15-ycf1_p3	GCGAAGTAAGCTTACGTACC	CGGCAGTCGTTTCAGTATGT	50.0	50.0	56.4	58.3	654-667 bp	1.0	65.7
rps15-ycf1_p4	GTGCGAAGTAAGCTTACGTAC	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.1	58.3	656-669 bp	1.0	63.6
rps15-ycf1_p5	TGCGAAGTAAGCTTACGTACC	GCAGTCGTTTCAGTATGTCG	47.6	50.0	58.1	57.0	653-666 bp	1.0	63.5