Hotspot

petA-psbJ

LSC · 63545-64568 · 1024 bp

Back to Ypsilandra

Pi 0.0334

MarkerSeek score 75.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 34
pi: 0.0334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGCATCCAAGGCCTTTT	CATATTCCGGATTGGGCTCA	45.0	50.0	58.1	57.4	1167-1168 bp	1.0	77.5
petA-psbJ_p2	GCATCCAAGGCCTTTTGTTC	CATATTCCGGATTGGGCTCA	50.0	50.0	57.9	57.4	1163-1164 bp	1.0	77.5
petA-psbJ_p3	ATTACGCATCCAAGGCCTTT	CATATTCCGGATTGGGCTCA	45.0	50.0	57.9	57.4	1168-1169 bp	1.0	77.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	1155-1156 bp	1.0	77.0
petA-psbJ_p5	TTACGCATCCAAGGCCTTTT	ATTCCGGATTGGGCTCATCT	45.0	50.0	58.1	58.9	1164-1165 bp	1.0	75.2