Hotspot

accD-psaI

LSC · 59024-59897 · 874 bp

Back to Ypsilandra

Pi 0.0103

MarkerSeek score 65.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 9
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTTGACCCAATCGTACCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1034-1058 bp	1.0	79.1
accD-psaI_p2	TTTGACCCAATCGTACCACG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1040-1064 bp	1.0	78.5
accD-psaI_p3	TGACCCAATCGTACCACGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.5	58.0	1032-1056 bp	1.0	77.1
accD-psaI_p4	TGACCCAATCGTACCACGTA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.5	57.9	1038-1062 bp	1.0	76.4
accD-psaI_p5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	1031-1055 bp	1.0	75.5