Hotspot

trnT_GGU-psbD

LSC · 31150-32182 · 1033 bp

Back to Ypsilandra

Pi 0.0184

MarkerSeek score 68.00

Primer available yes

Label trnT_GGU-psbD

Feature Metadata

label_name: trnT_GGU-psbD
feature_type: igs
region: LSC
length_bp: 1033
variable_sites: 19
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT_GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1184-1187 bp	1.0	79.4
trnT_GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	55.0	50.0	58.1	58.2	1185-1188 bp	1.0	79.4
trnT_GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1186-1189 bp	1.0	79.4
trnT_GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CACAAAAACGAAACGGTCCC	50.0	50.0	58.1	58.2	1187-1190 bp	1.0	79.2
trnT_GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCACAAAAACGAAACGGTCC	50.0	50.0	58.1	58.2	1188-1191 bp	1.0	79.2