Hotspot

trnE_UUC-trnT_GGU

LSC · 30499-31077 · 579 bp

Back to Ypsilandra

Pi 0.0138

MarkerSeek score 66.30

Primer available yes

Label trnE_UUC-trnT_GGU

Feature Metadata

label_name: trnE_UUC-trnT_GGU
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 8
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE_UUC-trnT_GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	720-724 bp	1.0	79.6
trnE_UUC-trnT_GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	699-703 bp	1.0	79.1
trnE_UUC-trnT_GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	696-700 bp	1.0	79.0
trnE_UUC-trnT_GGU_p4	CCGCTTCCTCCTTGAAAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	684-688 bp	1.0	78.7
trnE_UUC-trnT_GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	661-665 bp	1.0	78.7