Hotspot

trnS_GCU-trnG_UCC

LSC · 8225-8990 · 766 bp

Back to Ypsilandra

Pi 0.0132

MarkerSeek score 67.30

Primer available yes

Label trnS_GCU-trnG_UCC

Feature Metadata

label_name: trnS_GCU-trnG_UCC
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 10
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS_GCU-trnG_UCC_p1	CAATCCGTCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	873-891 bp	1.0	68.5
trnS_GCU-trnG_UCC_p2	AATCCGTCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	872-890 bp	1.0	68.5
trnS_GCU-trnG_UCC_p3	CAATCCGTCGCTTTAGTCCA	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.0	57.1	874-892 bp	1.0	66.6
trnS_GCU-trnG_UCC_p4	AATCCGTCGCTTTAGTCCAC	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.0	57.1	873-891 bp	1.0	66.6
trnS_GCU-trnG_UCC_p5	AGCAATCCGTCGCTTTAGTC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.4	56.4	875-893 bp	1.0	65.7