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psbK-psbI

LSC · 7461-7887 · 427 bp

Back to Ypsilandra

Pi 0.0141

MarkerSeek score 63.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 6
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTAGACGTTATGCCCATCA	GGATTGCGTCCTGGATCATT	50.0	50.0	57.5	58.0	601-611 bp	1.0	76.7
psbK-psbI_p2	GCTGCTGCAAGTTTTCGATG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.7	58.0	543-553 bp	1.0	75.5
psbK-psbI_p3	AGCTGCTGCAAGTTTTCGAT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.8	58.0	544-554 bp	1.0	74.7
psbK-psbI_p4	CTTTGTTTGGCAAGCTGCTG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.8	58.0	556-566 bp	1.0	74.5
psbK-psbI_p5	TCGTAGACGTTATGCCCATC	GGATTGCGTCCTGGATCATT	50.0	50.0	57.2	58.0	602-612 bp	1.0	74.4