Hotspot

trnQ_UUG-psbK

LSC · 6933-7274 · 342 bp

Back to Ypsilandra

Pi 0.0175

MarkerSeek score 63.50

Primer available yes

Label trnQ_UUG-psbK

Feature Metadata

label_name: trnQ_UUG-psbK
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 6
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ_UUG-psbK_p1	GATTCGAACCTCCGGGTAAC	GGGCAATTTGGCGAAGAAAA	55.0	45.0	58.1	57.8	483-489 bp	1.0	79.1
trnQ_UUG-psbK_p2	GGATTCGAACCTCCGGGTAA	GGGCAATTTGGCGAAGAAAA	55.0	45.0	59.2	57.8	484-490 bp	1.0	70.3
trnQ_UUG-psbK_p3	GGGTAACGGGACCAAAACC	GGGCAATTTGGCGAAGAAAA	57.9	45.0	58.4	57.8	470-476 bp	1.0	68.9
trnQ_UUG-psbK_p4	GGTAACGGGACCAAAACCC	GGGCAATTTGGCGAAGAAAA	57.9	45.0	58.4	57.8	469-475 bp	1.0	68.9
trnQ_UUG-psbK_p5	GAAGGATTCGAACCTCCGG	GGGCAATTTGGCGAAGAAAA	57.9	45.0	57.6	57.8	487-493 bp	1.0	68.8