Hotspot

trnK-UUU-rps16

LSC · 4103-4850 · 748 bp

Back to Ypsilandra

Pi 0.0161
MarkerSeek score 53.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
748
variable_sites
12
pi
0.0161
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ACCTTTCCGGATCAGTCGTA CAAAAGGTATGTTGCTGCCG 50.0 50.0 58.2 58.0 1561-1562 bp 1.0 79.9
trnK-UUU-rps16_p2 TACCTTTCCGGATCAGTCGT CAAAAGGTATGTTGCTGCCG 50.0 50.0 58.2 58.0 1562-1563 bp 1.0 79.9
trnK-UUU-rps16_p3 ACCTTTCCGGATCAGTCGTA GTTGCTGCCGTTTTGAGTTT 50.0 45.0 58.2 58.1 1551-1552 bp 1.0 79.1
trnK-UUU-rps16_p4 TACCTTTCCGGATCAGTCGT GTTGCTGCCGTTTTGAGTTT 50.0 45.0 58.2 58.1 1552-1553 bp 1.0 79.1
trnK-UUU-rps16_p5 ACCTTTCCGGATCAGTCGTA TGCTGCCGTTTTGAGTTTTG 50.0 45.0 58.2 58.1 1549-1550 bp 1.0 79.1