Hotspot

ccsA-ndhD

SSC · 114789-115057 · 269 bp

Back to Youngia

Pi 0.0349

MarkerSeek score 64.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 9
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGCGACTCTAGGCTTTCTT	TCAGTTGACAAGGTTGAAGGT	50.0	42.9	58.7	57.4	413-421 bp	1.0	64.8
ccsA-ndhD_p2	CAATTGTGGCGACTCTAGGC	TCAGTTGACAAGGTTGAAGGT	55.0	42.9	59.0	57.4	419-427 bp	1.0	62.7
ccsA-ndhD_p3	GCAATTGTGGCGACTCTAGG	TCAGTTGACAAGGTTGAAGGT	55.0	42.9	59.0	57.4	420-428 bp	1.0	62.7
ccsA-ndhD_p4	TGGCGACTCTAGGCTTTCT	TCAGTTGACAAGGTTGAAGGT	52.6	42.9	58.0	57.4	413-421 bp	1.0	62.5
ccsA-ndhD_p5	GTGGCGACTCTAGGCTTTC	TCAGTTGACAAGGTTGAAGGT	57.9	42.9	57.9	57.4	414-422 bp	1.0	62.3