Hotspot

ndhF-rpl32

SSC · 111605-112662 · 1058 bp

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Pi 0.0169

MarkerSeek score 60.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1058
variable_sites: 16
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	AATAAGACTCCCCACCACCA	50.0	50.0	58.0	57.9	1468-1580 bp	1.0	84.8
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	ATAAGACTCCCCACCACCAA	50.0	50.0	58.0	57.9	1467-1579 bp	1.0	84.8
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	CGAAGGTCGATGGGGAAAAT	50.0	50.0	58.0	58.0	1597 bp	0.5	59.4
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	CGAAGGTCGATGGGGAAAAT	45.0	50.0	58.0	58.0	1595 bp	0.5	59.3
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	CGAAGGTCGATGGGGAAAAT	45.0	50.0	58.0	58.0	1596 bp	0.5	59.3