Hotspot

accD-psaI

LSC · 58613-59416 · 804 bp

Back to Youngia

Pi 0.0189

MarkerSeek score 65.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 13
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	876-985 bp	1.0	71.1
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	875-984 bp	1.0	71.0
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	877-986 bp	1.0	71.0
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.0	58.0	876-985 bp	1.0	69.1
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	866-975 bp	1.0	69.1