Hotspot

ndhC-trnV-UAC

LSC · 50160-51390 · 1231 bp

Back to Youngia

Pi 0.0249

MarkerSeek score 77.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 29
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1319-1379 bp	1.0	82.2
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1321-1381 bp	1.0	81.3
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1324-1384 bp	1.0	81.3
ndhC-trnV-UAC_p4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.7	58.1	1320-1380 bp	1.0	79.1
ndhC-trnV-UAC_p5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1304-1364 bp	1.0	77.6