Hotspot

psaA-ycf3

LSC · 41273-42041 · 769 bp

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Pi 0.0212

MarkerSeek score 68.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 16
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	948-961 bp	1.0	78.5
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	950-963 bp	1.0	78.5
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	949-962 bp	1.0	76.7
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	883-896 bp	1.0	76.6
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	885-898 bp	1.0	76.6