Hotspot

trnG-GCC-trnfM-CAU

LSC · 35941-36125 · 185 bp

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Pi 0.0291

MarkerSeek score 71.40

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 5
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	250-263 bp	1.0	83.9
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	286-299 bp	1.0	83.4
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	244-257 bp	1.0	83.4
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	243-256 bp	1.0	82.8
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TTCAGTTCTTCTCTTCGGCG	50.0	50.0	57.8	57.9	308-321 bp	1.0	82.7