Hotspot

atpH-atpF

LSC · 26584-26959 · 376 bp

Back to Youngia

Pi 0.0188

MarkerSeek score 64.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 7
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTTATGGCCTGGTTGTAGCG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.3	57.9	465-467 bp	1.0	79.4
atpH-atpF_p2	TTTATGGCCTGGTTGTAGCG	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.3	57.9	496-498 bp	1.0	78.8
atpH-atpF_p3	TTTATGGCCTGGTTGTAGCG	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.3	57.9	466-468 bp	1.0	78.8
atpH-atpF_p4	TTTATGGCCTGGTTGTAGCG	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.3	58.2	467-469 bp	1.0	78.0
atpH-atpF_p5	CGGAGGGAAAAATACGAGGT	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.3	57.9	525-527 bp	1.0	75.7