Hotspot

trnE-UUC-rpoB

LSC · 12050-13035 · 986 bp

Back to Youngia

Pi 0.026
MarkerSeek score 68.00
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
986
variable_sites
25
pi
0.026
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.0 56.8 1111-1128 bp 1.0 65.7
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 1070-1087 bp 1.0 65.1
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1101-1118 bp 1.0 64.9
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1106-1123 bp 1.0 64.9
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.0 57.6 1101-1118 bp 1.0 64.3