Hotspot

rps16-trnQ-UUG

LSC · 6313-7291 · 979 bp

Back to Youngia

Pi 0.0194

MarkerSeek score 70.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 979
variable_sites: 19
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1051-1053 bp	1.0	79.9
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1066-1068 bp	1.0	78.0
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1065-1067 bp	1.0	78.0
rps16-trnQ-UUG_p4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1122-1124 bp	1.0	76.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1055-1057 bp	1.0	75.1