Hotspot

rps16

Genome · 1-991 · 991 bp

Back to Yoania

Pi 0.0388
MarkerSeek score 73.60
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
Genome
length_bp
991
variable_sites
69
pi
0.0388
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 CTTGTCGGTTTAGCCCCTTT CGTGCGACTTGAAGGACATA 50.0 50.0 58.1 58.0 916-1010 bp 1.0 87.3
rps16_p2 TTCCTTCTCTTCGGGATCGA CGTGCGACTTGAAGGACATA 50.0 50.0 57.9 58.0 813-907 bp 1.0 87.3
rps16_p3 CGGTTTAGCCCCTTTGTCAA CGTGCGACTTGAAGGACATA 50.0 50.0 58.4 58.0 911-1005 bp 1.0 84.9
rps16_p4 TTGTCGGTTTAGCCCCTTTG CGTGCGACTTGAAGGACATA 50.0 50.0 58.4 58.0 915-1009 bp 1.0 84.9
rps16_p5 AGTTCTTGTCGGTTTAGCCC CGTGCGACTTGAAGGACATA 50.0 50.0 57.8 58.0 920-1014 bp 0.75 74.6