Hotspot

ycf1-rps12

Genome · 37689-38713 · 1025 bp

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Pi 0.0408
MarkerSeek score 76.90
Primer available yes
Label ycf1-rps12

Feature Metadata

label_name
ycf1-rps12
feature_type
igs
region
Genome
length_bp
1025
variable_sites
75
pi
0.0408
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps12_p1 TCTCCCAGACATCGTAGAGA GGTAGCCTGCTTAAGTTCCC 50.0 55.0 56.6 58.0 1698-1785 bp 1.0 76.1
ycf1-rps12_p2 TCTCCCAGACATCGTAGAGA AGCCATACACTTCGCATGTT 50.0 45.0 56.6 57.9 1654-1741 bp 1.0 75.7
ycf1-rps12_p3 TCTCCCAGACATCGTAGAGA ACGGCTTTCCACAGAATTGT 50.0 45.0 56.6 58.0 2080-2170 bp 0.75 65.7
ycf1-rps12_p4 TCTCCCAGACATCGTAGAGA GGTTAGCCATACACTTCGCA 50.0 50.0 56.6 58.0 1658-1679 bp 0.75 64.4
ycf1-rps12_p5 TCTCCCAGACATCGTAGAGA ACCGGGCAAATCCTTAACTC 50.0 50.0 56.6 57.9 1499 bp 0.25 37.8