Hotspot

rrn5-ycf1

Genome · 32509-32951 · 443 bp

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Pi 0.0862
MarkerSeek score 81.30
Primer available yes
Label rrn5-ycf1

Feature Metadata

label_name
rrn5-ycf1
feature_type
igs
region
Genome
length_bp
443
variable_sites
64
pi
0.0862
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-ycf1_p1 CCACCTCAAGATGAGTGCTC TGCTGAGATCACGCATTGAA 55.0 45.0 58.0 57.9 1321-1608 bp 1.0 89.1
rrn5-ycf1_p2 CCACCTCAAGATGAGTGCTC TTGAATGCTGAGATCACGCA 55.0 45.0 58.0 57.9 1326-1613 bp 1.0 89.0
rrn5-ycf1_p3 ACCTGGGGCTGTAGTATGTT TGCTGAGATCACGCATTGAA 50.0 45.0 58.0 57.9 1571-1858 bp 1.0 87.7
rrn5-ycf1_p4 ACCTGGGGCTGTAGTATGTT TTGAATGCTGAGATCACGCA 50.0 45.0 58.0 57.9 1576-1863 bp 1.0 87.7
rrn5-ycf1_p5 TGGGGCTGTAGTATGTTCCA TGCTGAGATCACGCATTGAA 50.0 45.0 58.0 57.9 1568-1855 bp 1.0 87.7