Hotspot

rrn23-rrn4.5

Genome · 32042-32144 · 103 bp

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Pi 0.0647
MarkerSeek score 77.60
Primer available yes
Label rrn23-rrn4.5

Feature Metadata

label_name
rrn23-rrn4.5
feature_type
igs
region
Genome
length_bp
103
variable_sites
12
pi
0.0647
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23-rrn4.5_p1 CCACCTCAAGATGAGTGCTC TGATAAACGGCTCGTCTTGC 55.0 50.0 58.0 58.6 154-155 bp 1.0 82.9
rrn23-rrn4.5_p2 CCTCAAGATGAGTGCTCTCC TGATAAACGGCTCGTCTTGC 55.0 50.0 57.4 58.6 151-152 bp 1.0 78.5
rrn23-rrn4.5_p3 CCACCTCAAGATGAGTGCTC ATAAACGGCTCGTCTTGCC 55.0 52.6 58.0 58.2 152-153 bp 1.0 78.3
rrn23-rrn4.5_p4 CCACCTCAAGATGAGTGCTC GATAAACGGCTCGTCTTGCC 55.0 55.0 58.0 59.3 153-154 bp 1.0 77.4
rrn23-rrn4.5_p5 CACCTCAAGATGAGTGCTCT TGATAAACGGCTCGTCTTGC 50.0 50.0 56.9 58.6 153-154 bp 1.0 74.7