Hotspot

rrn16-rrn23

Genome · 28739-29210 · 472 bp

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Pi 0.113
MarkerSeek score 75.30
Primer available yes
Label rrn16-rrn23

Feature Metadata

label_name
rrn16-rrn23
feature_type
igs
region
Genome
length_bp
472
variable_sites
76
pi
0.113
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-rrn23_p1 GGCTGGATCACCTCCTTT GTCTCTGGGTGCCTAGGTAT 55.6 55.0 56.2 57.9 494-682 bp 1.0 60.6
rrn16-rrn23_p2 GGCTGGATCACCTCCTTT TCTCTGGGTGCCTAGGTATC 55.6 55.0 56.2 57.6 493-681 bp 1.0 58.2
rrn16-rrn23_p3 GGCTGGATCACCTCCTTT AAGCATTTCGTCGCTTGCTA 55.6 45.0 56.2 58.6 525-713 bp 1.0 56.8
rrn16-rrn23_p4 GGCTGGATCACCTCCTTT CTCTGGGTGCCTAGGTATCC 55.6 60.0 56.2 58.7 492-680 bp 1.0 56.0
rrn16-rrn23_p5 GGCTGGATCACCTCCTTT TGGGTGCCTAGGTATCCAC 55.6 57.9 56.2 58.1 489-677 bp 1.0 52.7