Hotspot

clpP-rps11

Genome · 11536-11811 · 276 bp

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Pi 0.0775
MarkerSeek score 80.60
Primer available yes
Label clpP-rps11

Feature Metadata

label_name
clpP-rps11
feature_type
igs
region
Genome
length_bp
276
variable_sites
38
pi
0.0775
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-rps11_p1 CTTTTGGAACACCAATGGGC GGATGTCGTCCCCCTAAAAA 50.0 50.0 57.8 57.2 321-526 bp 1.0 83.9
clpP-rps11_p2 TTTGGAACACCAATGGGCAT GGATGTCGTCCCCCTAAAAA 45.0 50.0 58.3 57.2 319-524 bp 1.0 83.1
clpP-rps11_p3 CTTTTGGAACACCAATGGGC ACATAATGGATGTCGTCCCC 50.0 50.0 57.8 57.1 328-533 bp 1.0 82.6
clpP-rps11_p4 TCCAGGACTCCGAAAAGGTA GGATGTCGTCCCCCTAAAAA 50.0 50.0 57.7 57.2 341-546 bp 1.0 82.4
clpP-rps11_p5 CCAGGACTCCGAAAAGGTAC GGATGTCGTCCCCCTAAAAA 55.0 50.0 57.6 57.2 340-545 bp 1.0 81.8