Hotspot

rpl33-rps18

Genome · 7678-7831 · 154 bp

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Pi 0.0649
MarkerSeek score 77.30
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
Genome
length_bp
154
variable_sites
17
pi
0.0649
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGAACTGAACTAAGAGCGCT TCGTCGTTTGTGGATCACTC 50.0 50.0 58.0 57.9 1162-1271 bp 1.0 89.6
rpl33-rps18_p2 GTTTTGGAGACCCACGTTCT TCGTCGTTTGTGGATCACTC 50.0 50.0 58.0 57.9 1184-1293 bp 1.0 89.4
rpl33-rps18_p3 GAAAAGGAGGTTCATGGCCA TCGTCGTTTGTGGATCACTC 50.0 50.0 58.1 57.9 979-1043 bp 1.0 89.2
rpl33-rps18_p4 TGAAAAGGAGGTTCATGGCC TCGTCGTTTGTGGATCACTC 50.0 50.0 58.1 57.9 980-1044 bp 1.0 89.2
rpl33-rps18_p5 AAAGGAGGTTCATGGCCAAG TCGTCGTTTGTGGATCACTC 50.0 50.0 58.1 57.9 977-1041 bp 0.75 78.0