Hotspot

trnC-GCA-trnY-GUA

Genome · 2342-2838 · 497 bp

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Pi 0.0476
MarkerSeek score 75.80
Primer available yes
Label trnC-GCA-trnY-GUA

Feature Metadata

label_name
trnC-GCA-trnY-GUA
feature_type
igs
region
Genome
length_bp
497
variable_sites
39
pi
0.0476
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnY-GUA_p1 CCCCTACTTGCTGCATCAAA CATAACCTTGAGACCACGGG 50.0 55.0 58.2 58.0 1273-1477 bp 1.0 86.4
trnC-GCA-trnY-GUA_p2 CCCCTACTTGCTGCATCAAA CTTGAGACCACGGGTTCAAA 50.0 50.0 58.2 58.0 1267-1471 bp 1.0 86.3
trnC-GCA-trnY-GUA_p3 CCCCTACTTGCTGCATCAAA GGAGGTAACGGGGATTCAAC 50.0 55.0 58.2 58.0 1113-1313 bp 1.0 86.2
trnC-GCA-trnY-GUA_p4 CCCCTACTTGCTGCATCAAA AAGGAGGTAACGGGGATTCA 50.0 50.0 58.2 58.0 1115-1315 bp 1.0 86.2
trnC-GCA-trnY-GUA_p5 CCCCTACTTGCTGCATCAAA AGGAGGTAACGGGGATTCAA 50.0 50.0 58.2 58.0 1114-1314 bp 1.0 86.2