Hotspot

rps16-trnQ-UUG

Genome · 992-1327 · 336 bp

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Pi 0.0701
MarkerSeek score 78.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
Genome
length_bp
336
variable_sites
40
pi
0.0701
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG CCTTACCACTTGGCCACG 50.0 61.1 58.0 58.0 390-490 bp 1.0 75.2
rps16-trnQ-UUG_p2 TATGTCCTTCAAGTCGCACG GTTGCCTTACCACTTGGCC 50.0 57.9 58.0 59.0 394-494 bp 1.0 75.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CCTTACCACTTGGCCACG 45.0 61.1 58.1 58.0 380-480 bp 1.0 74.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTTGCCTTACCACTTGGCC 45.0 57.9 58.1 59.0 384-484 bp 1.0 74.2
rps16-trnQ-UUG_p5 TATGTCCTTCAAGTCGCACG GCCTTACCACTTGGCCAC 50.0 61.1 58.0 58.3 391-491 bp 1.0 72.8