Hotspot

rpl16

LSC · 81699-83175 · 1477 bp

Back to Yinshania

Pi 0.00169
MarkerSeek score 60.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1477
variable_sites
5
pi
0.00169
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTTTTAATGCAAAGGACGAAGT TCAGCAATTCTATAAGGTTGA 34.8 33.3 57.0 52.3 1639-1645 bp 1.0 40.3
rpl16_p2 TCTTTTAATGCAAAGGACGAAGT TCAGCAATTCTATAAGGTTGAA 34.8 31.8 57.0 53.2 1639-1645 bp 1.0 40.3
rpl16_p3 CTCTTCTTTTAATGCAAAGGACGA TCAGCAATTCTATAAGGTTGA 37.5 33.3 57.8 52.3 1643-1649 bp 1.0 40.3
rpl16_p4 TCTCTTCTTTTAATGCAAAGGACG TCAGCAATTCTATAAGGTTGA 37.5 33.3 57.8 52.3 1644-1650 bp 1.0 40.3
rpl16_p5 TCTTCTTTTAATGCAAAGGACGA TCAGCAATTCTATAAGGTTGA 34.8 33.3 56.7 52.3 1642-1648 bp 1.0 40.3