Hotspot

psbA

LSC · 326-1387 · 1062 bp

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Pi 0

MarkerSeek score 29.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAAGGCTTATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	40.0	50.0	54.2	58.4	1168 bp	1.0	46.9
psbA_p2	AAGAAGGCTTATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	38.1	50.0	55.1	58.4	1169 bp	1.0	45.7
psbA_p3	AGAAGGCTTATATTGCTCGTT	ATTTGTGTGCTTGGGAGTCC	38.1	50.0	55.1	58.4	1168 bp	1.0	45.7
psbA_p4	AAGAAGGCTTATATTGCTCGTT	ATTTGTGTGCTTGGGAGTCC	36.4	50.0	55.9	58.4	1169 bp	1.0	44.0
psbA_p5	AGAAGGCTTATATTGCTCGTTT	ATTTGTGTGCTTGGGAGTCC	36.4	50.0	55.9	58.4	1168 bp	1.0	44.0