Hotspot

rpl32-trnL-CUA

SSC · 114366-114918 · 553 bp

Back to Yinshania

Pi 0.00488

MarkerSeek score 52.60

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 553
variable_sites: 4
pi: 0.00488
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCGAACCGAGATGCTTTAGC	47.6	50.0	57.2	58.1	713-738 bp	1.0	66.4
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	687-712 bp	1.0	66.1
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	680-705 bp	1.0	65.6
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	686-711 bp	1.0	65.5
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	685-710 bp	1.0	65.5