Hotspot

ndhF-rpl32

SSC · 113404-114206 · 803 bp

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Pi 0.00394

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 803
variable_sites: 6
pi: 0.00394
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	ACGATGTCCAATATCCCTTCC	45.0	47.6	58.0	57.2	953-1020 bp	1.0	68.7
ndhF-rpl32_p2	TGTTGCTGTCGGAAAAAGGA	CGATGTCCAATATCCCTTCCT	45.0	47.6	58.0	56.9	952-1019 bp	1.0	66.4
ndhF-rpl32_p3	TGTTGCTGTCGGAAAAAGGA	AACGATGTCCAATATCCCTTCC	45.0	45.5	58.0	57.9	954-1021 bp	1.0	66.2
ndhF-rpl32_p4	TGTTGCTGTCGGAAAAAGGA	AAAGCTTTCAACGATGTCCA	45.0	40.0	58.0	55.9	963-1030 bp	1.0	65.8
ndhF-rpl32_p5	TGTTGCTGTCGGAAAAAGGA	AAGCTTTCAACGATGTCCAA	45.0	40.0	58.0	55.9	962-1029 bp	1.0	65.8