Hotspot

petA-psbJ

LSC · 62958-63956 · 999 bp

Back to Yinshania

Pi 0.0035

MarkerSeek score 51.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 7
pi: 0.0035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.0	58.1	1193-1207 bp	1.0	79.1
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.2	58.1	1190-1204 bp	1.0	78.0
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1188-1202 bp	1.0	76.6
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.4	58.1	1187-1201 bp	1.0	76.2
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.5	58.1	1183-1197 bp	1.0	75.8