Hotspot

psbM-trnD-GAC

LSC · 28890-29926 · 1037 bp

Back to Yinshania

Pi 0.00161

MarkerSeek score 51.40

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 3
pi: 0.00161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1154-1161 bp	1.0	68.2
psbM-trnD-GAC_p2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1150-1157 bp	1.0	67.5
psbM-trnD-GAC_p3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.3	58.5	1153-1160 bp	1.0	66.0
psbM-trnD-GAC_p4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.6	58.5	1151-1158 bp	1.0	65.3
psbM-trnD-GAC_p5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.5	58.5	1153-1160 bp	1.0	64.8