Hotspot

rpoB-trnC-UGC

LSC · 26406-27445 · 1040 bp

Back to Yinshania

Pi 0.000481

MarkerSeek score 59.80

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 1
pi: 0.000481
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1128-1191 bp	1.0	78.7
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTATTGAGGCGGC	55.0	50.0	57.9	58.3	1150-1213 bp	1.0	77.3
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1110-1173 bp	1.0	76.5
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1129-1192 bp	1.0	72.7
rpoB-trnC-UGC_p5	TGTTCCCTCTTTTCCATCCC	AAGTCTGTTATTGAGGCGGC	50.0	50.0	57.1	58.3	1151-1214 bp	1.0	71.3