Hotspot

trnG-GGA-trnR-AGA

LSC · 9474-9626 · 153 bp

Back to Yinshania

Pi 0.0131

MarkerSeek score 56.90

Primer available yes

Label trnG-GGA-trnR-AGA

Feature Metadata

label_name: trnG-GGA-trnR-AGA
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 4
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GGA-trnR-AGA_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	270-276 bp	1.0	50.1
trnG-GGA-trnR-AGA_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	276-282 bp	1.0	50.0
trnG-GGA-trnR-AGA_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	290-296 bp	1.0	49.7
trnG-GGA-trnR-AGA_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	278-284 bp	1.0	49.6
trnG-GGA-trnR-AGA_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	271-277 bp	1.0	49.1