Hotspot

trnK-AAA-rps16

LSC · 4297-5039 · 743 bp

Back to Yinshania

Pi 0.00389

MarkerSeek score 63.20

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 5
pi: 0.00389
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AACTCTACCGCAGATCTGGA	CGCTCAACCAACAAGAACAG	50.0	50.0	57.9	57.6	918-933 bp	1.0	77.5
trnK-AAA-rps16_p2	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	850-865 bp	1.0	77.4
trnK-AAA-rps16_p3	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	849-864 bp	1.0	77.4
trnK-AAA-rps16_p4	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	836-851 bp	1.0	75.1
trnK-AAA-rps16_p5	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	861-876 bp	1.0	74.2