Hotspot

trnH-CAC-psbA

LSC · 77-325 · 249 bp

Back to Yinshania

Pi 0.0147

MarkerSeek score 53.30

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 7
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	307 bp	1.0	80.3
trnH-CAC-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	308 bp	1.0	80.1
trnH-CAC-psbA_p3	AATCCACTTGGCTACATCCG	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.7	58.1	300 bp	1.0	78.8
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	374 bp	1.0	77.7
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	375 bp	1.0	77.5